GA-10細(xì)胞
生長狀態(tài)： 懸浮生長
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
ATCC Number： CRL-2392?
相關(guān)**： **瘤
數(shù)量： 大量
細(xì)胞類型： B**細(xì)胞
年限： 41 years
是否是腫瘤細(xì)胞： 1
物種來源： 人
Designations: GA-10
Depositors: AP Rapoport
GA-10細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens deposited as human
Morphology: lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Cellular Products: immunoglobulin; kappa light chain
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GA-10細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: CD10 +, CD19 +, CD20 +, CD40 + (10%), CD45 +, CD77 + (90%), CD34 -
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,20
Cytogenetic Analysis: The reported karyoype was 46, XY, dup(1) (pter to q32::q21 to qter), t(8;14) (q24.1; q32), -10, +der(10)t(Y;10)(q12:q26), -21, +der (21) t (?; 21) (?; p11)., In addition a few cells lacked a second chromosome 21 and carried an additional der(21) t(?;21) (?;q22). GA-10細(xì)胞The Vh segment in this cell line was Vh4-34 (Vh 4.21).
Age: 41 years
Gender: male
Ethnicity: Caucasian, White
Comments: GA-10 was derived in 1996 from lymphoma cells circulating in the peripheral blood of a patient with a clinically aggressive, chemo-refractory Burkitt's Lymphoma.
The patient had been treated unsuccessfully with cyclophosphamide, hydroxydaunomycin, vincristine, and prednisone (CHOP chemotherapy).
The cells express relatively high levels of CD77 (approximately 90%); CD77 is also known as Burkitt's lymphoma antigen (BLA).
GA-10 cells exhibit sensitivity to Shiga Toxin (Stx) in toxicity studies.
This cell line is slightly more sensitive to doxorubicin and etoposide than HL-60 leukemia cells.
Flavopiridol, a potent inhibitor of cell cycle progression through G1 and G2, produces apoptosis of the GA-10 cells at relatively low concentrations of 20-30 nM.
GA-10 cells are negative for Epstein-Barr virus (EBV).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Preservation: GA-10細(xì)胞culture medium 95%; DMSO, 5%
Doubling Time: 24 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derived from same cell line:ATCC CRL-2393
derived from same cell line:ATCC CRL-2394
