Cates-1B細胞
細胞形態(tài)： 混合型
ATCC Number： HTB-104?
相關(guān)**： 其他**
器官來源： 睪丸
運輸方式： 凍存運輸
年限： 34 years
是否是腫瘤細胞： 1
物種來源： 人
數(shù)量： 大量
生長狀態(tài)： 貼壁生長
Designations: Cates-1B
Depositors: J Fogh
Cates-1B細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: mixed
Source: Organ: testis
Disease: embryonal carcinoma
Derived from metastatic site: lymph node
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Cates-1B細胞Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Isolation: Isolation date: 1973
DNA Profile (STR): Amelogenin: XY
CSF1PO: 10
D13S317: 12
D16S539: 12, 13
D5S818: 12, 13
D7S820: 7, 12
THO1: 6
TPOX: 8
vWA: 16, 17
Cytogenetic Analysis: modal number = 46; range = 40 to 53.
This is a pseudodiploid human cell line with the modal chromosome number of 46, occurring in 56% of cells. The rate of polyploidy was 1.9%. Cates-1B細胞There were no constitutive marker chromosomes. The N2 was trisomic. There was a high incidence of Y chromosome losses, which contributed to the high number of cells with 46 chromosomes.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1-2
Age: 34 years
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: Cates-1B細胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Interval: Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
