HCC1599細胞

數量： 大量

組織來源： duct

細胞形態(tài)： 上皮樣

年限： TNM stage IIIA, grade 3

是否是腫瘤細胞： 1

物種來源： 人

ATCC Number： CRL-2331?

相關**： 導管癌

運輸方式： 凍存運輸

生長狀態(tài)： 懸浮生長，多細胞聚集

器官來源： **

Designations: HCC1599

HCC1599細胞Depositors: AF Gazdar, AK Virmani

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: mammary gland; breast

Tissue: duct

Tumor Stage: TNM stage IIIA, grade 3

Disease: primary ductal carcinoma

Cellular Products: Epithelial glycoprotein 2 [EGP2]; cytokeratin 19

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. HCC1599細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.

Isolation: Isolation date: March 28, 1998

Applications: The cells are poorly differentiated.

The cells are negative for expression of Her2-neu and for expression of p53.

The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).

HCC1599 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2), and for cytokeratin 19.

Receptors: estrogen receptor, negative

progesterone receptor, negative

Oncogene: her2/neu -, p53 -

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 11

D16S539: 12,13

D5S818: 12

HCC1599細胞D7S820: 10,11

THO1: 9.3

TPOX: 8

vWA: 17

Cytogenetic Analysis: multiploid. The cell population has several ploidy indices.

Age: 44 years *****

Gender: female

Ethnicity: Caucasian, White

Comments: The HCC1599 cell line was initiated on March 28, 1995, and took 10 months to establish.

The tumor was classified as a TNM stage IIIA, grade 3, invasive ductal carcinoma. Information regarding lymph node metastasis is not available.

The cells are poorly differentiated.

The cells are negative for expression of Her2-neu and for expression of p53.

The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).

HCC1599 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2), and for cytokeratin 19.

An EBV-transformed lymphoblastoid cell line (HCC1599 BL) from the same patient is available as ATCC CRL-2332.

Propagation: HCC1599細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

normal (or near-normal) cell line established from the same patient:ATCC CRL-2332

purified DNA:ATCC CRL-2331D

purified RNA:ATCC CRL-2331R

cell pellet:ATCC CRL-2331P

References: 38266: Gazdar AF, et al. HCC1599細胞Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771