SW 1573細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-2170?

相關(guān)**： 其他**

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

器官來(lái)源： 肺

數(shù)量： 大量

年限： 44 years

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

細(xì)胞形態(tài)： 上皮樣

Designations: SW 1573 [SW-1573, SW1573]

Depositors: W McCombs

SW 1573細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: lung

Disease: alveolar cell carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: These cells are distributed for research purposes only. The Scott and White Clinic releases the line subject to the following: 1) The cells or products derived from them must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. SW 1573細(xì)胞Commercial interests are the exclusive property of the Scott and White Clinic. 2) Any proposed commercial use of these cells or products produced by them must first be negotiated with the Scott and White Clinic, 2401 S. 31 Street, Temple, Texas 76508. Telephone (817) 774-2432

Antigen Expression: Blood Type O; Rh +

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,12

D13S317: 11

D16S539: 12,14

D5S818: 12,13

D7S820: 9,11

THO1: 6,9.3

TPOX: 8,11

vWA: 20

Age: 44 years

Gender: female

Ethnicity: Caucasian

SW 1573細(xì)胞Comments: The SW 1573 line was established in 1977 by A. Leibovitz from an alveolar cell carcinoma.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 100%

Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

References: 22551: Wright WC, et al. SW 1573細(xì)胞Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474