U-118 MG細(xì)胞

數(shù)量： 大量

器官來源： 大腦

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

年限： classified as grade IV as of 2007

細(xì)胞形態(tài)： 混合型

ATCC Number： HTB-15?

相關(guān)**： 其他**

是否是腫瘤細(xì)胞： 1

物種來源： 人

Designations: U-118 MG

U-118 MG細(xì)胞Depositors: J Ponten

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: mixed

Source: Organ: brain

Tumor Stage: classified as grade IV as of 2007

Disease: glioblastoma; astrocytoma

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Tumorigenic: Yes

Antigen Expression: Blood Type A, Rh+; HLA Aw24, A28, B12, Bw47

DNA Profile (STR): U-118 MG細(xì)胞Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 9

D16S539: 12,13

D5S818: 11

D7S820: 9

THO1: 6

TPOX: 8

vWA: 18

Isoenzymes: AK-1, 1-2

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 2

PGM3, 2

Age: 50 years

Gender: male

Ethnicity: Caucasian

Comments: NOTE: U-118 MG細(xì)胞The two glioblastoma cell lines, U-118 MG (HTB-15) and U-138 MG (HTB-16), reportedly from different individuals have identical VNTR and similar STR patterns.

U-118 MG and U-138 MG are very similar cytogenetically and share at least six derivative marker chromosomes.

This is one of a number of cell lines derived from malignant gliomas (see also ATCC HTB-14 and ATCC HTB-16) by J. Ponten and associates from 1966 to 1969.

Mycoplasma contamination was eliminated in 1987 by treatment with BM-Cycline over a six week culture period.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapot temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22159: Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744

22536: Fogh J, et al. U-118 MG細(xì)胞Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

23094: Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221

23128: Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. PubMed: 4313504

23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

23260: Bluestein HG. Neurocytotoxic antibodies in serum of patients with systemic lupus erythematosus. Proc. Natl. Acad. Sci. USA 75: 3965-3969, 1978. PubMed: 279013

32276: Cairns P, et al. U-118 MG細(xì)胞Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760