Hs 700T細(xì)胞

數(shù)量： 大量

年限： 61 years

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 1

物種來源： 人

生長狀態(tài)： 貼壁生長

細(xì)胞形態(tài)： 上皮樣

ATCC Number： HTB-147?

相關(guān)**： 腺癌

Designations: Hs 700T

Depositors: RB Owens

Hs 700T細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Disease: adenocarcinoma

Derived from metastatic site: pelvis

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Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10

D13S317: 8

D16S539: 10,13

D5S818: 11, 12

D7S820: 9, 11

THO1: 9.3

Hs 700T細(xì)胞TPOX: 8, 12

vWA: 18, 19

Cytogenetic Analysis: modal number = 58 to 59; range = 55 to 107.

The cell line is aneuploid human male, with most chromosome counts in the hypertetraploid range. A substantial (30%) population of hyperdiploid to hypotriploid cells is present. In this population, normal chromosome N7 is absent, while chromosomes N3, N4, N8, N18, and N21 are clearly under-represented with respect to the copy numbers of other normal chromosomes. Chromosomes N2 and N20 tend to be over-represented, while modal number = 58 to 59; range = 55 to 107.

Chromosome N15 is clearly so. Twelve marker chromosomes, some with modifications, are present. A double-minute chromosome was seen in one of 32.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 0

PGM1, 1

PGM3, 1

Age: 61 years

Gender: male

Ethnicity: Caucasian

Comments: Hs 700T was established by R. Owens, et al. at the Naval Biosciences Laboratory from an adenocarcinoma metastatic to the pelvis of a 61 year old male Caucasian.

Hs 700T細(xì)胞The mucin producing, primary tumor was thought to have originated from the intestines or pancreas.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:Hs 700T細(xì)胞ATCC 30-2020

References: 22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412

22543: Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258

23221: Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033