HCT-8 (HRT-18)細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來源： 人
ATCC Number： CCL-244?
相關(guān)**： 其他**
細(xì)胞形態(tài)： 上皮樣
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 貼壁生長
器官來源： 結(jié)腸
數(shù)量： 大量
年限： 67 years *****
Designations: HCT-8 [HRT-18]
Depositors: A Sobrero
HCT-8 (HRT-18)細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Disease: ileocecal colorectal adenocarcinoma
Cellular Products: carcinoembryonic antigen (CEA) 0.5 ng/10 exp6 cells/10 days; alkaline phosphatase; keratin
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Applications: The cells are positive for keratin by immunoperoxidase staining.
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
HCT-8 (HRT-18)細(xì)胞D7S820: 10,12,11.3
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19
Isoenzymes: AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 67 years *****
Gender: male
Comments: The HCT-8 line is identical to the HRT-18 cell line.
The cells are positive for keratin by immunoperoxidase staining.
Propagation: HCT-8 (HRT-18)細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, rinse cell sheet twice with 0.25% trypsin, 0.02% EDTA in Hanks' BSS and incubate at 37C with the trypsin, EDTA for 10 to 15 minutes. Remove trypsin, EDTA, disperse cells in fresh medium, centrifuge, resuspend cells in medium and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001
Recommended serum: ATCC 30-2040
0.25% (w/v) Trypsin ? 0.53mM EDTA in Hank?s BSS (w/o Ca++, Mg ++): ATCC 30-2101
Cell culture tested DMSO: ATCC 4-X
Derivative: ATCC CRL-11663
References: 1052: Tompkins WA, et al. Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J. Natl. HCT-8 (HRT-18)細(xì)胞Cancer Inst. 52: 1101-1110, 1974. PubMed: 4826581
26064: Nelson-Rees WA, et al. Distinctive banded marker chromosomes of human tumor cell lines. Int. J. Cancer 16: 74-82, 1975. PubMed: 1058173
32376: White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293
