SJRH30細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

年限： 17 years

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

組織來(lái)源： muscle

細(xì)胞形態(tài)： 成纖維樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： CRL-2061?

相關(guān)**： 橫紋肌肉瘤

SJRH30細(xì)胞Designations: SJCRH30 [RC13, RMS 13, SJRH30]

Depositors: DN Shapiro

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Tissue: muscle

Disease: rhabdomyosarcoma

Derived from metastatic site: bone marrow

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Oncogene: SJRH30細(xì)胞gli + (amplified 30 fold), N-myc + (not amplified)

DNA Profile (STR): D5S818: 12, 13

D13S317: 11

D7S820: 10

D16S539: 12

THO1: 9, 9.3

TPOX: 8, 11

vWA: 17, 18

CSF1PO: 10, 11

Amelogenin: XY

Age: 17 years

Gender: male

Ethnicity: Caucasian

Comments: SJRH30細(xì)胞The line was established from cells from the bone marrow of a child with rhabdomyosarcoma.

The cells show ultrastructural elements of primitive skeletal muscle differentiation.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended

Medium Renewal: Twice per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22379: Oliner JD, et al. Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358: 80-83, 1992. PubMed: 1614537

23076: Roberts WM, et al. Amplification of the gli gene in childhood sarcomas. Cancer Res. 49: 5407-5413, 1989. PubMed: 2766305

23518: Douglass EC, et al. SJRH30細(xì)胞A specific chromosomal abnormality in rhabdomyosarcoma [published erratum appears in Cytogenet Cell Genet 1988;47(4):following 232]. Cytogenet. Cell Genet. 45: 148-155, 1987. PubMed: 3691179