TOV-112D細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

細(xì)胞形態(tài)： 上皮樣

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

年限： grade 3, stage IIIC

ATCC Number： CRL-11731?

相關(guān)**： 其他**

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

器官來(lái)源： 卵巢

Designations: TOV-112D

Depositors: University of Montreal

TOV-112D細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens deposited as human

Morphology: epithelial

Source: Organ: ovary

Tumor Stage: grade 3, stage IIIC

Disease: primary malignant adenocarcinoma; endometrioid carcinoma

Cellular Products: keratin [49408]

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Tumorigenic: Yes

Oncogene: her2/neu +, p53 (mutated, Arg --> His mutation at exon 6, codon 175)

DNA Profile (STR): TOV-112D細(xì)胞Amelogenin: X

CSF1PO: 12

D13S317: 8

D16S539: 9,12

D5S818: 10

D7S820: 9,10

THO1: 6

TPOX: 8,11

vWA: 18

Cytogenetic Analysis: 52, XX, add(X)(p22), +add(1)(p22), +add(1)(p22), +2, +9, +12, add(15)(p11), +17 [49408]

Age: 42 years *****

Gender: female

Comments: This cell line was initiated in October of 1992 from a patient with early onset ovarian cancer. The patient was of French-Canadian descent with an unknown family history of ovarian cancer. [49408]

Unlike TOV-21G (ATCC CRL-11730) and OV-90 (ATCC CRL-11732), the TOV-112D cell line does not exhibit a deletion at chromosome 3p24. [42090]

TOV-112D細(xì)胞Propagation: ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 15%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.05% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: recommended serum:ATCC 30-2020

purified DNA:ATCC CRL-11731

References: 42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998

49408: Provencher DM, et al. TOV-112D細(xì)胞Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993