NCI-H841細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

數(shù)量： 大量

年限： stage L

ATCC Number： CRL-5845?

相關(guān)**： 其他**

生長(zhǎng)狀態(tài)： 混合型生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 肺

Designations: NCI-H841 [H841]

Depositors: AF Gazdar, JD Minna

NCI-H841細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed: adherent and suspension, Cultures grow as floating aggregates of round cell clusters and as attached epithelial-like cells.

Organism: Homo sapiens

Morphology:

Source: Organ: lung

Tumor Stage: stage L

Disease: carcinoma; variant small cell lung cancer

Derived from metastatic site: lymph node

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NCI-H841細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11

D13S317: 12

D16S539: 13

D5S818: 11,12

D7S820: 10,13

THO1: 6,9

TPOX: 11,12

vWA: 16,17

Cytogenetic Analysis: t(2p,3p), t(3q,9q)

Age: 51 years

Gender: male

Ethnicity: NCI-H841細(xì)胞Caucasian, White

Comments: The line was established in October 1984.

The patient received prior chemotherapy and radiation therapy.

The patient was a smoker.

70.

Propagation: ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum

The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium

0.005 mg/ml Insulin

0.01 mg/ml Transferrin

30nM Sodium selenite (final conc.)

10 nM Hydrocortisone (final conc.)

10 nM beta-estradiol (final conc.)

extra 2mM L-glutamine (for final conc. of 4.5 mM)

5% fetal bovine serum (final conc.)

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended

Medium Renewal: NCI-H841細(xì)胞Add fresh medium as cell density increases.

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium.

Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.