TE 381.T細(xì)胞
數(shù)量： 大量
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
ATCC Number： CRL-7763?
相關(guān)**： 橫紋肌肉瘤
運(yùn)輸方式： 凍存運(yùn)輸
年限： 7 years
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
組織來(lái)源： connective and soft tissue
細(xì)胞形態(tài)： 成纖維樣
Designations: TE 381.T [RD114-B]
TE 381.T細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Tissue: connective and soft tissue
Disease: rhabdomyosarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis: TE 381.T細(xì)胞modal number = 46; range = 36 to 90
Age: 7 years
Gender: female
Ethnicity: Caucasian
Comments: This line was derived by passing TE 32.T (ATCC CRL-7731) through a fetal cat.
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . TE 381.T細(xì)胞We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
TE 381.T細(xì)胞Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
