ME-180細胞

ATCC Number： HTB-33?

相關**： 上皮細胞癌

器官來源： 宮頸

生長狀態(tài)： 貼壁生長

運輸方式： 凍存運輸

數(shù)量： 大量

是否是腫瘤細胞： 1

物種來源： 人

細胞形態(tài)： 上皮樣

年限： 66 years

Designations: ME-180

Depositors: JA Sykes

Biosafety Level: 2 [Cells contain human papilloma virus ]

ME-180細胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: cervix

Disease: epidermoid carcinoma

Derived from metastatic site: omentum

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Applications: Desmosomal attachments were observed between cells in monolayer culture and cytoplasmic tonofilaments were noted.

This line was derived from a highly invasive squamous cell carcinoma with irregular cell clusters and no significant keratinization.

Tumorigenic: Yes

Oncogene: p53 +; pRB +

Antigen Expression: Blood Type A; Rh+; HLA A1, A11, B5(+/-), B40

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 11,13

ME-180細胞D16S539: 12,13

D5S818: 12

D7S820: 9,10

THO1: 8,9.3

TPOX: 8,10

vWA: 15,17

Cytogenetic Analysis: hyperdiploid to hypohexaploid with abnormalities including dicentrics, fragmentation and markers; unstable; (P63) hypotriploid with abnormalities including dicentrics and acrocentric fragments, large submetacentric and large subtelocentric markers

Isoenzymes: AK-1, 1

ES-D, 1-2

G6PD, B

GLO-I, 2

PGM1, 1-2

PGM3, 1

Age: 66 years

Gender: female

Ethnicity: Caucasian

Comments: ME-180細胞This line was derived from a highly invasive squamous cell carcinoma with irregular cell clusters and no significant keratinization.

Desmosomal attachments were observed between cells in monolayer culture and cytoplasmic tonofilaments were noted.

Contamination with Mycoplasma arginini was detected and eliminated in 1970.

Growth of ME-180 cells is inhibited by tumor necrosis factor alpha (TNF alpha).

The cells contain human papillomavirus (HPV) DNA with greater homology to HPV-39 than HPV-18.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007

recommended serum:ATCC 30-2020

References: 21405: ME-180細胞Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111

22520: Sykes JA, et al. Some properties of a new epithelial cell line of human origin. J. Natl. Cancer Inst. 45: 107-122, 1970. PubMed: 4317734

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

22995: Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153

23180: Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

23238: Reuter S, et al. Characterization of a novel human papillomavirus DNA in the cervical carcinoma cell line ME180. J. Virol. 65: 5564-5568, 1991. PubMed: 1716694

23324: Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218

29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

32285: van Dijk MA, et al. A functional assay in yeas for the human estrogen receptor displays wild-type and variant estrogen receptor messenger RNAs present in breast carcinoma. Cancer Res. 57: 3478-3485, 1997. PubMed: 9270016

32299: St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830