Hs 766T細(xì)胞

器官來(lái)源： 胰腺

細(xì)胞形態(tài)： 上皮樣

ATCC Number： HTB-134?

相關(guān)**： 腫瘤

年限： 46 years

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

運(yùn)輸方式： 凍存運(yùn)輸

Designations: Hs 766T

Depositors: RB Owens

Hs 766T細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: pancreas

Disease: carcinoma

Derived from metastatic site: lymph node

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Applications: transfection host (Roche Transfection Reagents)

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 8,11

D16S539: 9,12

Hs 766T細(xì)胞D5S818: 11

D7S820: 10

THO1: 6,9.3

TPOX: 8

vWA: 18

Cytogenetic Analysis: (P11) hyperdiploid with mode = 60; banded markers; lacking Y chromosomes

Isoenzymes: AK-1, 1

ES-D, 1-2

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1-2

PGM3, 1

Age: 46 years

Gender: male

Ethnicity: Caucasian

Comments: The Hs 766T line was isolated by R. Owens et al. at the Naval Biosciences Laboratory in 1973 from a pancreatic carcinoma metastatic to a lymph node.

The epithelial nature of the cell strain selected was confirmed by electron microscopy as microvilli and desmosomes were observed.

Hs 766T細(xì)胞Tests conducted at an early passage indicated that the cells exhibited many properties observed only in tumor lines. The karyotype was abnormal and the cells formed colonies in Methocel and on contact inhibited monolayers.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412

22543: Smith HS. Hs 766T細(xì)胞In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258

23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

26328: . Prevention and detection of cancer. vol. 2New York: Marcel Dekker; 1978.