HS-Sultan細(xì)胞

年限： 7

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： kappa light chain

生長狀態(tài)： 懸浮生長，多細(xì)胞聚集

細(xì)胞類型： B**細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來源： 人

運輸方式： 凍存運輸

ATCC Number： CRL-1484?

相關(guān)**： **瘤

Designations: HS-Sultan

Depositors: NS Harris

Isotype: kappa light chain

HS-Sultan細(xì)胞Biosafety Level: 2 [Cells contain EBV DNA viral sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens

Morphology: lymphoblast

Source: Disease: Burkitt's lymphoma

Cell Type: B lymphocyte;

Cellular Products: immunoglobulin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: CD11a +; CD19 +; CD20 +; CD28 -; CD38 +; CD49e +

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 12

D16S539: 10,11

D5S818: 12

D7S820: 8,10

THO1: 7,9

TPOX: 6,8

HS-Sultan細(xì)胞vWA: 15,19

Cytogenetic Analysis: This is a hyperdiploid human cell line. The modal chromosome number was 48, occurring in 80% of cells, and the rate of polyploid cells was 3.4%. Three marker chromosomes are common to all cells. M1 and M2 [der(14)t(8;14) (q24;q32)] were probably derived from the balanced translocation between del(8) (p21.1;p21.3) and N14. The third marker, M3, is ?der(8)t(8;?) (p21.1;?). Most cells had three copies of N7. Both normal X and Y were clearly identifiable.

Age: 7

Gender: male

Ethnicity: Black, African

Comments: The cells are EBNA positive (EBNA +).

Although deposited with the ATCC as a plasmacytoma cell line, DNA fingerprinting has shown this line to be a derivative of Jiyoye (ATCC CCL-87), a Burkitt's lymphoma cell line.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml.Maintain cell density between 3 X 10(5) and 3 X 10(6) viable cells/ml.Do not allow the cell density to exceed 4 X 10(6) cells/ml.

HS-Sultan細(xì)胞Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

parental cell line:ATCC CCL-87

References: 22894: Harris NS. Plasma cell surface antigen on human blood lymphocytes. Nature 250: 507-509, 1974. PubMed: 4549735

23445: . . J. Immunol. 118: 1480-1482, 1976.

24402: . . Proceedings of the Seventh Leukocyte Culture Conference : 15-28, 1973.

43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. HS-Sultan細(xì)胞PubMed: 7579375