1A2細(xì)胞

細(xì)胞類型： 其他細(xì)胞類型

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來源： 人

細(xì)胞形態(tài)： **樣

ATCC Number： CRL-8119?

運(yùn)輸方式： 凍存運(yùn)輸

Designations: 1A2

Depositors: Genetic Systems Corp.

1A2細(xì)胞Biosafety Level: 2 [CELLS CONTAIN HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Cell Type: lymphoblast B lymphocyte; Epstein-Barr virus (EBV) transformed

Cellular Products: Epstein-Barr virus (EBV)

Permits/Forms: 1A2細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: This line was derived from the GM1500 lymphoma line.

The cells are resistant to 6-thioguanine.

The cells can be used as a source of infectious EBV, and can be used as driving the cell in"cell driven" EBV transformation of human B cells.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 1 mM sodium pyruvate and 2 mM L-glutamine, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: 1A2細(xì)胞Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 2 X 10(5) and 1 X 10(6) cells/ml.

Interval: every 3 days

Medium Renewal: Every 3 days

Preservation: Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 24 hrs (when cells are in logarithmic growth)

References: 3532: Lostrom ME. Cell-driven viral transfer in eukaryotes. US Patent 4,464,465 dated Aug 7 1984

23264: Croce CM, et al. 1A2細(xì)胞Chromosomal location of the genes for human immunoglobulin heavy chains. Proc. Natl. Acad. Sci. USA 76: 3416-3419, 1979. PubMed: 114999