SH-4細(xì)胞

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來源： 人

生長狀態(tài)： 貼壁生長

細(xì)胞形態(tài)： 其他

ATCC Number： CRL-7724?

相關(guān)**： 黑色素瘤

年限： 60 years

運(yùn)輸方式： 凍存運(yùn)輸

器官來源： 皮膚

Designations: SH-4

SH-4細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: mixture of spindle-shaped and epithelial-like cells

Source: Organ: skin

Disease: melanoma

Derived from metastatic site: pleural effusion

Cellular Products: melanin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 9,12

D16S539: 11,12

SH-4細(xì)胞D5S818: 11,12

D7S820: 13

THO1: 8,9.3

TPOX: 8,11

vWA: 16,17

Cytogenetic Analysis: modal number = 53; range = 45 to 56

Age: SH-4細(xì)胞60 years

Gender: female

Ethnicity: Caucasian

Comments: Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.

Please see the NBL Repository description.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

References: 22395: Seman G, et al. SH-4細(xì)胞Establishment of a cell line (SH-4) from pleural effusion of a patient with melanoma. In Vitro 11: 205-211, 1975. PubMed: 1176156