GA-10 (Clone 20)細(xì)胞
生長狀態(tài)： 懸浮生長
ATCC Number： CRL-2394?
相關(guān)**： **瘤
數(shù)量： 大量
運輸方式： 凍存運輸
細(xì)胞形態(tài)： **樣
年限： 41 years
細(xì)胞類型： B**細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來源： 人
Designations: GA-10 (Clone 20)
GA-10 (Clone 20)細(xì)胞Depositors: AP Rapoport
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens deposited as human
Morphology: lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
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Antigen Expression: CD77 + (80-90%)
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,10
THO1: 9,9.3
GA-10 (Clone 20)細(xì)胞TPOX: 8,11
vWA: 16,20
Age: 41 years
Gender: male
Ethnicity: Caucasian, White
Comments: GA-10 (Clone 20) is a subclone of the GA-10 (ATCC CRL-2392) cell line derived by culturing the parental line on semi-solid medium conditioned by a feeder layer.
GA-10 was derived in 1996 from lymphoma cells circulating in the peripheral blood of a patient with a clinically aggressive, chemo-refractory Burkitt's Lymphoma.
The patient had been treated unsuccessfully with cyclophosphamide, hydroxydaunomycin, vincristine, and prednisone (CHOP chemotherapy).
This clone of cells also expresses high levels of CD77 (80-90%); CD77 is also known as Burkitt's lymphoma antigen (BLA).
GA-10 (Clone-20) cells exhibit marked sensitivity to Shiga Toxin (Stx) in cytotoxicity studies.
Propagation: GA-10 (Clone 20)細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derived from same cell line:ATCC CRL-2392
derived from same cell line:GA-10 (Clone 20)細(xì)胞ATCC CRL-2393
