143B PML BK TK細(xì)胞

年限： 13 year old

運(yùn)輸方式： 凍存運(yùn)輸

器官來源： 骨

ATCC Number： CRL-8304?

相關(guān)**： 骨肉瘤

是否是腫瘤細(xì)胞： 1

物種來源： 人

細(xì)胞形態(tài)： 成纖維樣

數(shù)量： 大量

生長狀態(tài)： 貼壁生長

143B PML BK TK細(xì)胞Designations: 143B PML BK TK

Depositors: Wistar Institute

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: bone

Disease: osteosarcoma

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DNA Profile (STR): 143B PML BK TK細(xì)胞Amelogenin: X

CSF1PO: 12

D13S317: 12

D16S539: 10,13

D5S818: 13

D7S820: 11,12

THO1: 6

TPOX: 11

vWA: 18

Age: 13 year old

Gender: female

Ethnicity: Caucasian

Comments: Contains a thymidine kinase (TK+) plasmid (PMBKTK).

143B PML BK TK細(xì)胞Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with HAT (0.1 mM hypoxanthine, 400 nM aminopterin, 0.16 mM thymidine), 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Related Products: plasmid in bacteria:ATCC 39369

parental cell line:143B PML BK TK細(xì)胞ATCC CRL-8303