KHOS-321H細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 貼壁生長
年限： 13 years
數(shù)量： 大量
ATCC Number： CRL-1546?
相關(guān)**： 骨肉瘤
器官來源： 骨
是否是腫瘤細(xì)胞： 1
物種來源： 人
Designations: KHOS-312H
Depositors: JS Rhim
KHOS-321H細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology:
Source: Organ: bone
Disease: osteosarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: No
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 12
KHOS-321H細(xì)胞D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
Age: 13 years
Gender: female
Ethnicity: Caucasian
Comments: The growth properties of KHOS-312H are similar to HOS (ATCC CRL-1543).
This line is a revertant of ATCC CRL-1544 that no longer has a rescuable Kirsten murine sarcoma virus genome.
Note: An inconsistency in our records was discovered late in 2010 concerning the human osteosarcoma cell line, KHOS-312H. Due to an ATCC catalog transfer error, this cell line has been referred to as both KHOS-312H and KHOS-321H by ATCC and in the literature. The name originally supplied to ATCC by the depositor was KHOS-312H.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. KHOS-321H細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin-0.53 mM EDTAsolution, remove trypsin and let the culture sit at 37C for 10 to 12 minutes or until cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 days.
References: 22503: Yang YH, et al. Reversion of Kirsten sarcoma virus transformed human cells: elimination of the sarcoma virus nucleotide sequences. J. Gen. Virol. 43: 447-451, 1979. PubMed: 225428
26080: Cho HY, et al. Revertants of human cells transformed by murine sarcoma virus. Science 194: 951-953, 1976. PubMed: 62397
26082: Cho HY, Rhim JS. Cycloheximide-dependent reversion of human cells transformed by MSV and chemical carcinogen. Science 205: 691-693, 1979.KHOS-321H細(xì)胞 PubMed: 223242
