MES-SA細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

年限： 56 years

ATCC Number： CRL-1976?

相關(guān)**： 其他**

細(xì)胞形態(tài)： 成纖維樣

器官來(lái)源： **

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

Designations: MES-SA

MES-SA細(xì)胞Depositors: BI Sikic

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: uterus

Disease: uterine sarcoma

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Isolation: Isolation date: 1980

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 11,13

MES-SA細(xì)胞D16S539: 11,12

D5S818: 13

D7S820: 7,11

THO1: 6

TPOX: 8,11

vWA: 18

Cytogenetic Analysis: This is a hypo-diploid human cell line with the modal chromosome number of 45 in 48% of cells examined. The rate of polyploidy was 2.7%. There were five or six marker chromosomes present in each cell. Whereas t(5q6p), 10q+, and 14q+ markers were common in all cells examined, the chromosome combination of either double copy N21 lacking M5 (66.7%) or t(21qter--->C--->?)(M5)/single copy N21/minute metacentric (33.3%) distinguishes the two coexisting clones. Both X chromosomes were normal.

Age: 56 years

Gender: female

Ethnicity: Caucasian

Comments: The MES-SA cell line was established from a surgical tumor specimen obtained at the time of hysterectomy.

The tumor was described as a poorly differentiated uterine sarcoma.

Initially, the cells were grown in soft agar, MES-SA細(xì)胞and later they were transferred to muliwell plates.

The nonepithelial origin of the cells was supported by ultrastructural studies and the absence of staining for mucin.

The cells are sensitive to a number of chemotherapeutic agents including doxorubicin, dactinomycin, mitomycin C, taxol and bleomycin.

They are resistant to vinblastine, dacarbazine, cisplatin, melphalan, vincristine, methotrexate and etoposide.

The multiple drug resistant cell line MES-SA/Dx5 (ATCC CRL-1977) was established from MES-SA cells which were grown in the presence of doxorubicin.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Growth Conditions: Newborn calf serum may be substituted for fetal bovine serum.

Subculturing: Protocol: Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 ml double distilled water). Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: MES-SA細(xì)胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 22 to 24 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007

recommended serum:ATCC 30-2020

derivative:ATCC CRL-1977

derivative:ATCC CRL-2274

References: 23048: Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344