Hs 936.T(C1)細(xì)胞
ATCC Number： CRL-7687?
相關(guān)**： 黑色素瘤
運輸方式： 凍存運輸
數(shù)量： 大量
器官來源： 皮膚
細(xì)胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
是否是腫瘤細(xì)胞： 1
Hs 936.T(C1)細(xì)胞物種來源： 人
Designations: Hs 936.T(C1)
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: skin
Disease: melanoma
Hs 936.T(C1)細(xì)胞Cellular Products: melanin
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Gender: male
Comments: Clone of ATCC CRL-7686.
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Hs 936.T(C1)細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: 1:2 to 1:4
Medium Renewal: every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO 5%
Storage temperature:Hs 936.T(C1)細(xì)胞 liquid nitrogen vapor
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
