Hs 93.T細(xì)胞
ATCC Number： CRL-7062?
相關(guān)**： 纖維肉瘤
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
細(xì)胞形態(tài)： 成纖維樣
年限： 12 years
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
數(shù)量： 大量
組織來(lái)源： connective and soft tissue
Hs 93.T細(xì)胞運(yùn)輸方式： 凍存運(yùn)輸
Designations: Hs 93.T
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Tissue: connective and soft tissue
Disease: fibrosarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Hs 93.T細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis: modal number = 46; range = 43 to 46
Age: 12 years
Gender: male
Ethnicity: Caucasian
Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Hs 93.T細(xì)胞Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:Hs 93.T細(xì)胞ATCC 30-2020
