Hs 821.T細(xì)胞

ATCC Number： CRL-7554?

相關(guān)**： 巨細(xì)胞肉瘤

是否是腫瘤細(xì)胞： 1

物種來源： 人

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

器官來源： 骨

運(yùn)輸方式： 凍存運(yùn)輸

年限： 31 years

Hs 821.T細(xì)胞細(xì)胞形態(tài)： 成纖維樣

Designations: Hs 821.T

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: bone

Disease: giant cell sarcoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Hs 821.T細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 31 years

Gender: female

Ethnicity: Caucasian

Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: Hs 821.T細(xì)胞air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:Hs 821.T細(xì)胞ATCC 30-2020