Hs 729細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： HTB-153?

數(shù)量： 大量

相關(guān)**： 橫紋肌肉瘤

年限： 74 years

細(xì)胞形態(tài)： 成纖維樣

組織來(lái)源： muscle

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

Hs 729細(xì)胞Designations: Hs 729 [Hs 729T]

Depositors: RB Owens

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Tissue: muscle

Disease: rhabdomyosarcoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Hs 729細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10

D13S317: 11,12

D16S539: 11

D5S818: 11,12

D7S820: 8,9

THO1: 6,9.3

TPOX: 11

vWA: 16,17

Isoenzymes: G6PD, B

Age: 74 years

Gender: male

Ethnicity: Caucasian

Hs 729細(xì)胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).

Add fresh medium, aspirate and dispense into new flasks.

Subculture every 6 to 8 days.

Preservation: Culture medium, 95%; Hs 729細(xì)胞DMSO, 5%