Hs 688(B).T細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

器官來(lái)源： **結(jié)

ATCC Number： CRL-7426?

相關(guān)**： 黑色素瘤

細(xì)胞形態(tài)： 成纖維樣

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

年限： 69 years

Designations: Hs 688(B).T

Hs 688(B).T細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: lymph node

Disease: melanoma

Derived from metastatic site: inguinal lymph node

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Hs 688(B).T細(xì)胞Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 12,13

D16S539: 9,13

D5S818: 8,11

D7S820: 10,11

THO1: 7,9

TPOX: 8,11

vWA: 15,18

Cytogenetic Analysis: modal number = 46; range = 45 to 50

Age: 69 years

Gender: male

Ethnicity: Caucasian

Comments: Hs 688(B).T細(xì)胞Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:Hs 688(B).T細(xì)胞ATCC 30-2020

derived from same individual:ATCC CRL-7425