Hs 602細(xì)胞
是否是腫瘤細(xì)胞: 1
物種來源: 人
ATCC Number: HTB-142?
相關(guān)**: **瘤
器官來源: 其他
數(shù)量: 大量
生長狀態(tài): 懸浮生長
運(yùn)輸方式: 凍存運(yùn)輸
細(xì)胞形態(tài): 其他
Hs 602細(xì)胞Designations: Hs 602
Depositors: P Arnstein
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: single spherical or small clusters
Source: Organ: cervical lymph node
Disease: lymphoma
Permits/Forms: Hs 602細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Cytogenetic Analysis: no Y chromosome noted
Isoenzymes: ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 1-2
Gender: female
Propagation:Hs 602細(xì)胞 ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 3 X 10 exp5 cells/ml and maintain at between 4 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22287: Arnstein P, et al. Propagation of human tumors in antithymocyte serum-treated mice. J. Natl. Cancer Inst. 52: 71-84, 1974. PubMed: 4544026
22550: Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Hs 602細(xì)胞Cancer Inst. 65: 715-718, 1980. PubMed: 6932523