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產(chǎn)品資料

Hs 184.T細胞

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產(chǎn)品名稱: Hs 184.T細胞
產(chǎn)品型號: Hs 184.T
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

Hs 184.T細胞應(yīng)如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術(shù)不當、操作室環(huán)境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細胞來源和培養(yǎng)基配制是減低污染之*好方法。Hs 184.T細胞何時須更換培養(yǎng)基?視細胞生長密度而定,或遵照細胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


Hs 184.T細胞  的詳細介紹

Hs 184.T細胞

是否是腫瘤細胞: 1

物種來源: 人

運輸方式: 凍存運輸

年限: 21 years *****

數(shù)量: 大量

細胞形態(tài): 成纖維樣

生長狀態(tài): 貼壁生長

ATCC Number: CRL-7134?

相關(guān)**: 骨肉瘤

器官來源: 骨

Designations: Hs 184.T

Hs 184.T細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast


Source: Organ: bone

Disease: osteosarcoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11

D13S317: 11

D16S539: 11,12

D5S818: 11,13

Hs 184.T細胞D7S820: 9,11

THO1: 9,9.3

TPOX: 12

vWA: 15,16

Age: 21 years *****

Gender: male

Ethnicity: Caucasian

Comments: Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Hs 184.T細胞Remove and discard culture medium.

Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting

Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002

Recommended serum: ATCC 30-2020

0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): Hs 184.T細胞ATCC 30-2101

Cell culture tested DMSO: ATCC 4-X

滬公網(wǎng)安備 31011702004356號

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