NCI-H295細(xì)胞
年限： 48 years
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 懸浮生長
數(shù)量： 大量
細(xì)胞形態(tài)： 上皮樣
器官來源： 腎上腺
ATCC Number： CRL-10296?
相關(guān)**： 其他**
組織來源： cortex
是否是腫瘤細(xì)胞： 1
物種來源： 人
NCI-H295細(xì)胞Designations: NCI-H295 [H295]
Depositors: AF Gazdar
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, with some loosely adherent cells, suspension with some loosely attached cells
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: adrenal gland
Tissue: cortex
Disease: adrenocortical carcinoma
Cellular Products: steroid hormones
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NCI-H295細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 13
D16S539: 11
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8
vWA: 17,18
Cytogenetic Analysis: modal number = 62; range = 55 to 64; hypertriploid. Twenty-three marker chromosomes were common to most cells: der(2)t(2;18)(p25;p11), del(3)(q21), der(10)t(10;13)(p15;q11), der(7)t(7;7)(q36;p13), del(7)(q11),; der(8)t(7;8)(p13;p23), del(8)(p12), del(9)(p22), del(9)(p13), del(11)(p12), del(12)(p12), t(13q18q) and 11 others. The del(9)(p13) was paired; normal N13 was absent. N15 had three copies and N22 had four copies.; NCI-H295細(xì)胞A total of 65 marker chromosomes were detected in 38 metaphases examined.; A minor subline that occurred in about 16% of the cells was characterized by the presence of del(M5)(7p11) replacing M5, by the single N17 (instead of paired N17) and by the presence of an additional M42.
Age: 48 years
Gender: female
Ethnicity: Black
Propagation: ATCC complete growth medium: HITES medium supplemented with 2% fetal bovine serum
HITES medium with 2% fetal bovine serum is formulated at the ATCC as follows:
Dulbecco's medium : Ham's F12, 50:50 mix (ATCC 30-2006)
Insulin 0.005 mg/ml
Transferrin 0.01 mg/ml
Sodium selenite 30 nM
Hydrocortisone 10 nM
beta-estradiol 10 nM
HEPES 10 mM
L-glutamine 2 mM (in addition to that in the base medium)
fetal bovine serum 2%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Cells grow as floating aggregates of round cells with some loosely attached cells. NCI-H295細(xì)胞Shake the flask to dislodge the cells and transfer to a new flask.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with an additional 8% fetal bovine serum and 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
References: 23082: Gazdar AF, et al. Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis. Cancer Res. 50: 5488-5496, 1990. PubMed: 2386954
