我的性奴的肉玩具1一17心奴,天堂a免费视频在线观看,久久久久久欧美精品无码,26uuu国产精品色噜噜

產(chǎn)品資料

pBK-CMV

如果您對(duì)該產(chǎn)品感興趣的話,可以
產(chǎn)品名稱: pBK-CMV
產(chǎn)品型號(hào):
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔

簡(jiǎn)單介紹

pBK-CMV的各批次質(zhì)粒菌株發(fā)貨前均經(jīng)過(guò)嚴(yán)格的多重驗(yàn)證,如存在質(zhì)量問(wèn)題,請(qǐng)?jiān)谑盏疆a(chǎn)品的三個(gè)月內(nèi)通知我司。收到pBK-CMV后請(qǐng)短暫離心,取2μl轉(zhuǎn)化至對(duì)應(yīng)感受態(tài)中,挑取單克隆重新提取質(zhì)粒后使用。


pBK-CMV  的詳細(xì)介紹

pBK-CMV載體基本信息

載體名稱: pBK-CMV  Phagemid vector ;pBK-CMV
質(zhì)粒類型: 真核原核雙系統(tǒng)表達(dá)載體;克隆載體;噬粒載體
高拷貝/低拷貝: 高拷貝
克隆方法: 多克隆位點(diǎn),限制性內(nèi)切酶
啟動(dòng)子: 原核啟動(dòng)子lac;真核啟動(dòng)子CMV
載體大小: 4518bp
5' 測(cè)序引物及序列: --
3' 測(cè)序引物及序列: --
載體標(biāo)簽: --
載體抗性: Kanamycin.html' target='_blank'>卡那霉素
篩選標(biāo)記: 新霉素Neomycin 和卡那霉素Kanamycin
備注: 用藍(lán)白斑法篩選原核細(xì)胞陽(yáng)性克隆。
產(chǎn)品目錄號(hào): --
穩(wěn)定性: --
組成型/誘導(dǎo)型: 真核組成型表達(dá),原核IPTG誘導(dǎo)型表達(dá)
病毒/非病毒: 非病毒

pBK-CMV載體質(zhì)粒圖譜和多克隆位點(diǎn)信息

pBK-CMV載體圖譜



pBK-CMV 多克隆位點(diǎn)

pBK-CMV 載體特征

pBK-CMV載體簡(jiǎn)介

pBK-CMV Phagemid Vector
The pBK-CMV vector allows you to express proteins in both E. coli and mammalian model systems and saves you valuable time by not having to subclone into an additional vector.  In mammalian cells, the expression is driven by the CMV promoter and is therefore constitutively expressed.  In E. coli, the lac promoter drives high-level expression in the presence of the inducer IPTG, and allows minimal expression in the absence of inducer.

? Constitutive expression in eukaryotic cells
? Inducible protein expression in prokaryotes
? Rescued from ZAP Express lambda vector


The pBK-CMV phagemid vector is a cloning vector derived from a high-copy-number pUC-based plasmid. This vector allows expression in both eukaryotic and prokaryotic systems. Eukaryotic expression is driven by the cytomegalovirus (CMV) immediate early promoter in the pBK-CMV phagemid vector. Stable clone selection in eukaryotic cells is made possible with G418 by the presence of the neomycin- and kanamycin-resistance gene, which is driven by the SV40 early promoter with thymidine kinase (TK) transcription termination and polyadenylation signals. In the pBK-CMV phagemid vector, prokaryotic expression is driven by the lac promoter, which is repressed in the presence of the LacI protein and is inducible by IPTG. In bacteria expressing the lacZΔM15 mutation and lacI, colonies containing vector without insert will be blue in the presence of X-gal and IPTG. Kanamycin-resistant colonies containing vector with insert will be white and can express the inserted gene as a fusion protein. 

Inserts should be cloned within the polylinker cloning sites for prokaryotic expression. For eukaryotic expression one can clone either within the polylinker or between the Nhe I site at the 5′ end of the lac promoter and a polylinker cloning site at the 3′ end. Removing the upstream lac sequences from the eukaryotic transcript by cloning between the Nhe I site and the polylinker sites has been shown to give elevated eukaryotic expression with test inserts.  

The pBK–CMV phagemid vector has an extensive polylinker in an SK orientation (the Sac I site is the closest restriction site to the lacZ promoter, and the Kpn I site is the farthest restriction site from the lacZ promoter). The polylinker contains 17 unique restriction enzyme recognition sites, organized with alternating 5′ and 3′ overhangs to allow serial exonuclease III/mung bean nuclease deletions.2 Sites with compatible restriction overhangs, such as Spe I–Xba I and Sal I–Xho I, have been placed on opposite sides of the EcoR I site to allow unidirectional cloning in both the sense and antisense orientations with the ZAP Express vectors and the pBK–CMV phagemid vector. The (–) orientation of the f1 intergenic (IG) region in pBK-CMV allows rescue of antisense single-stranded DNA (ssDNA) relative to thelacZ transcript. This ssDNA can be used for dideoxynucleotide sequencing (Sanger method) or site-specific mutagenesis. Flanking the polylinker are T3 and T7 RNA polymerase promoters that can be used to synthesize RNA in vitro. The choice of promoter used to initiate transcription determines which strand of the DNA insert will be transcribed. 

pBK-CMV載體序列

hz-6384R GIDRP88  生長(zhǎng)抑制和分化相關(guān)蛋白抗體
hz-6385R Gas1  生長(zhǎng)休止特定蛋白1抗體
hz-6386R MDFIC  肌原調(diào)節(jié)抑制蛋白抗體
hz-6387R MEI-1  減數(shù)分裂缺陷蛋白1抗體
hz-6388R MTBP/MDM2BP  雙微體2癌基因結(jié)合蛋白抗體
hz-6389R NIRF/RNF107  環(huán)指蛋白107抗體
hz-6390R Necdin  生長(zhǎng)抑制蛋白NDN抗體
hz-6391R RPRD1A/P15RS  細(xì)胞周期依賴性激酶抑制相關(guān)蛋白抗體
hz-6392R PCNP  PEST含核蛋白抗體
hz-6393R PLK4/STK18  絲氨酸/蘇氨酸蛋白激酶18
hz-6394R Quiescin Q6/QSOX1  巰基氧化酶1抗體
hz-6395R LEU5/RFP2  白血病相關(guān)蛋白5抗體
hz-6396R SAPK3/MAPK12  應(yīng)激活化蛋白激酶3抗體(絲裂原活化蛋白激酶12)
hz-6397R SCGB3A1  結(jié)合珠蛋白家族3A1抗體
hz-6398R SEI2/SERTAD2  調(diào)控周期蛋白依賴蛋白激酶SEI2抗體
hz-6399R SIPA1  信號(hào)誘導(dǎo)增殖相關(guān)蛋白1抗體
hz-0177R AGER  晚期糖基化終末產(chǎn)物特異性受體抗體
hz-5159R Fast skeletal Myosin/MRLC2  骨骼肌肌球蛋白輕鏈2抗體
hz-6400R TFDP3  轉(zhuǎn)錄因子DP3抗體(肝癌相關(guān)抗原661)
hz-6401R BMP1  骨形態(tài)發(fā)生蛋白1/膠原C蛋白肽鏈內(nèi)切酶抗體
hz-6402R SMARCA2/BRM  ATP依賴解旋酶SMARCA2抗體
hz-6403R Ferritin Light Chain  鐵蛋白輕鏈抗體
hz-6404R CECR5  貓眼綜合征染色體候選基因5抗體
hz-6405R CEE  跨膜結(jié)構(gòu)域邊緣蛋白CEE抗體
hz-6406R CESK1  分子伴侶CESK1蛋白抗體

滬公網(wǎng)安備 31011702004356號(hào)

信阳市| 穆棱市| 桃园市| 宁陕县| 永川市| 开封县| 永仁县| 当涂县| 琼中| 马山县| 东台市| 中西区| 洪泽县| 云阳县| 新竹县| 平原县| 承德县| 临夏县| 铜川市| 罗甸县| 西安市| 五家渠市| 仪征市| 永安市| 菏泽市| 琼海市| 吐鲁番市| 安庆市| 新绛县| 科技| 伊宁市| 武宣县| 上高县| 阜南县| 北安市| 剑阁县| 汶上县| 天祝| 辰溪县| 灵川县| 涿鹿县|