pCMV-Green-Renilla-Luc載體基本信息
載體名稱: | pCMV-Green-Renilla-Luc |
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質(zhì)粒類型: | 哺乳動物表達(dá)載體;綠色熒光素酶報告載體 |
高拷貝/低拷貝: | 高拷貝 |
克隆方法: | 限制性內(nèi)切酶,多克隆位點 |
啟動子: | CMV |
載體大小: | 5860 bp |
5' 測序引物及序列: | -- |
3' 測序引物及序列: | -- |
載體標(biāo)簽: | -- |
載體抗性: | 氨芐青霉素 |
篩選標(biāo)記: | 嘌呤霉素(Puromycin) |
克隆菌株: | DH5α 等 |
宿主細(xì)胞(系): | 常規(guī)細(xì)胞系(293、CV-1、CHO等) |
備注: | -- |
產(chǎn)品目錄號: | 16153 |
穩(wěn)定性: | 穩(wěn)表達(dá) |
組成型/誘導(dǎo)型: | 組成型 |
病毒/非病毒: | 非病毒 |
pCMV-Green Renilla Luc 載體簡介 The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the CMV (Cytomegalovirus) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters. pCMV-Green Renilla Luc 載體特征: Strong (CMV) constitutive promoter for co-transfection and normalization
Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
High-copy pUC bacterial DNA replication origin