pMCS-Green-Renilla-Luc載體基本信息
載體名稱: | pMCS-Green-Renilla-Luc 或 pMCS-Green Renilla Luc 、 pMCS-Renilla Luc |
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質(zhì)粒類型: | 哺乳動(dòng)物表達(dá)載體;綠色熒光素酶報(bào)告載體 |
高拷貝/低拷貝: | 高拷貝 |
克隆方法: | 限制性內(nèi)切酶,多克隆位點(diǎn) |
啟動(dòng)子: | 無(wú) |
載體大小: | 5263 bp |
5' 測(cè)序引物及序列: | -- |
3' 測(cè)序引物及序列: | -- |
載體標(biāo)簽: | -- |
載體抗性: | 氨芐青霉素 |
篩選標(biāo)記: | 嘌呤霉素(Puromycin) |
克隆菌株: | DH5α 等 |
宿主細(xì)胞(系): | 常規(guī)細(xì)胞系(293、CV-1、CHO等) |
備注: | -- |
產(chǎn)品目錄號(hào): | 16152 |
穩(wěn)定性: | 穩(wěn)表達(dá) |
組成型/誘導(dǎo)型: | 組成型 |
病毒/非病毒: | 非病毒 |
pMCS-Green Renilla Luc 載體簡(jiǎn)介 The Thermo Scientific pMCS-Renilla Luc vector is a multiple cloning site plasmid to designed to accept a promoter sequence for study of gene regulation using the intracellular Renilla luciferase reporter. pMCS-Renilla Luc 載體特征: Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
High-copy pUC bacterial DNA replication origin
The Green Renilla Luc Vectors contain a gene cloned from the sea pansy, Renilla reniformis. The gene encodes a bioluminescent Green Renilla luciferase (36kDa). The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.
These vectors are subject to a limited use label license.