pMCS-Red-Firefly-Luc載體簡介 Thermo Scientific pMCS-Red Firefly Luc is a multiple cloning site plasmid designed to accept a promoter sequence for the study of gene regulation using the intracellular red firefly luciferase reporter with excellent light intensity..
Features of pMCS-Red Firefly Luc:
? Intracellular Red Firefly Luciferase gene, optimized for high expression in mammalian systems
? Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
? Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
? Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
? High-copy pUC bacterial DNA replication origin
? Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization
Features of Red Firefly Luc:
? Red Firefly Luc Vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.
? The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.
? The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively.
? These pCMV and pTK constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.
