pGL4.50載體簡介
The pGL4.50[luc2/CMV/Hygro] Vector encodes the luciferase reporter gene luc2 (Photinus pyralis), which has been codon optimized for mammalian expression. This vector also is engineered with fewer consensus regulatory sequences for reduced backgrounds and a decreased risk of anomalous transcription. This vector contains the following features: luc2 reporter gene for expression in mammalian cells; CMV promoter for high translational expression; SV40 late poly(A) signal sequence is positioned downstream of luc2 to provide efficient transcription termination and mRNA polyadenylation; Binding region for RV primer 3 and RV primer 4; Synthetic poly(A) signal/transcription start site; Synthetic Hygromycin B-resistance gene for mammalian cell selection of the plasmid; Plasmid replication origin; Ampr gene for bacterial selection for vector amplification.