pGL4.10載體基本信息
載體名稱: | pGL4.10 |
---|---|
質(zhì)粒類型: | 哺乳細胞表達載體;信號通路報告載體 |
高拷貝/低拷貝: | -- |
啟動子: | 無 |
克隆方法: | 多克隆位點,限制性內(nèi)切酶 |
載體大小: | 4242bp |
5' 測序引物及序列: | -- |
3' 測序引物及序列: | -- |
載體標簽: | -- |
載體抗性: | Ampicillin |
篩選標記: | -- |
備注: | Promoterless vector for measuring the activity of promoter and enhancer sequences with a luciferase assay. |
產(chǎn)品目錄號: | E6651 |
穩(wěn)定性: | -- |
組成型: | -- |
病毒/非病毒: | -- |
The pGL4.10[luc2] Vector(a–d) encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for
high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory
sequences and a synthetic gene, which has been codon optimized for mammalian expression.
The pGL4.10[luc2] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning
of a promoter of choice.