The pGL4.30[luc2P/NFAT-RE/Hygro] Vector(a–f) contains an NFAT response element (NFAT-RE) that
drives the transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase
sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The
luc2P gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than
the protein encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow
selection in E. coli and a mammalian selectable marker for hygromycin resistance.