pGL4.75載體基本信息
載體名稱: | pGL4.75、 pGL4.75[hRluc/CMV] |
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質(zhì)粒類型: | 哺乳細胞表達;信號通路報告 |
高拷貝/低拷貝: | -- |
啟動子: | CMV |
克隆方法: | 多克隆位點,限制性內(nèi)切酶 |
載體大小: | 4281 bp |
5' 測序引物及序列: | -- |
3' 測序引物及序列: | -- |
載體標簽: | -- |
載體抗性: | Ampicillin |
篩選標記: | -- |
備注: | pGL4.75含有hRluc報告基因,是信號通路報告載體。 |
產(chǎn)品目錄號: | E693A |
穩(wěn)定性: | -- |
組成型: | 組成型 |
病毒/非病毒: | 非病毒 |
The pGL4.75[hRluc/CMV] Vector(a-c) encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.
The pGL4.75[hRluc/CMV] Vector contains the hRluc reporter gene and a CMV immediate-early enhancer/promoter and can be used as an expression control or a co-reporter vector.