pcDNA3.1/His A載體基本信息
載體名稱(chēng): | pcDNA3.1/His A , pcDNA3.1/HisA |
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質(zhì)粒類(lèi)型: | 哺乳動(dòng)物細(xì)胞表達(dá)載體 |
啟動(dòng)子: | CMV |
表達(dá)水平: | 高 |
克隆方法: | 多克隆位點(diǎn),限制性?xún)?nèi)切酶 |
載體大小: | 5514 bp |
5' 測(cè)序引物: | pcDNA3.1-F:CTAGAGAACCCACTGCTTAC |
3' 測(cè)序引物: | pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
載體標(biāo)簽: | N-His, N-EK |
載體抗性: | Ampicillin 氨芐 |
篩選標(biāo)記: | Neomycin (G418) |
備注: |
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產(chǎn)品目錄號(hào): | V385-20 |
穩(wěn)定性: | 瞬表達(dá) Transient |
組成型: | 組成型 Constitutive |
病毒/非病毒: | 非病毒 |
pcDNA3.1/His A, B, and C are 5.5 kb vectors derived from pcDNA3.1 and designed for high-level expression and purification of recombinant proteins in mammalian hosts. The vectors are supplied in three reading frames to facilitate in frame cloning with a polyhistidine metal-binding tag. The human cytomegalovirus (CMV) immediate-early promoter provides high-level expression in a wide range of mammalian cells. In addition, the vector replicates episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/His lacZ, is the pcDNA3.1/His B vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the N-terminal peptide. pcDNA3.1/His lacZ is included for use as a positive control for transfection, expression, and purification in the cell line of choice.