pBI-CMV1 is a mammalian bidirectional expression vector that allows the constitutive expression of two proteins of interest. Protein expression is driven by one of two constitutively active, minimal human cytomegalovirus promoters, PminCMV1 and PminCMV2, located just upstream of two independent multiple cloning sites (MCS 1 and MCS 2, respectively). To allow propagation and selection in E. coli, the vector also contains a ColE1 origin of replication and an ampicillin resistance gene (Ampr).


pBI-CMV1 is designed to simultaneously and constitutively express two genes of interest. Each gene must be cloned into a different MCS, and contain both an initiation codon and a stop codon. The pBI-CMV1 vector can be transfected into mammalian cells using any standard transfection method.