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產(chǎn)品資料

pIRES2-AcGFP1

如果您對該產(chǎn)品感興趣的話,可以
產(chǎn)品名稱: pIRES2-AcGFP1
產(chǎn)品型號:
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

pIRES2-AcGFP1的各批次質(zhì)粒菌株發(fā)貨前均經(jīng)過嚴格的多重驗證,如存在質(zhì)量問題,請在收到產(chǎn)品的三個月內(nèi)通知我司。收到pIRES2-AcGFP1后請短暫離心,取2μl轉(zhuǎn)化至對應(yīng)感受態(tài)中,挑取單克隆重新提取質(zhì)粒后使用。


pIRES2-AcGFP1  的詳細介紹

pIRES2-AcGFP1載體基本信息

載體名稱: pIRES2-AcGFP1
質(zhì)粒類型: 哺乳細胞表達載體 ; 雙順反子載體;熒光報告載體
高拷貝/低拷貝: 高拷貝
克隆方法: 多克隆位點,限制性內(nèi)切酶
啟動子: CMV
載體大小: 5307 bp
5' 測序引物及序列: --
3' 測序引物及序列: --
載體標簽: 綠色熒光蛋白AcGFP1
載體抗性: Kanamycin.html' target='_blank'>卡那霉素Kanamycin
篩選標記: Neomycin
備注: pIRES2-AcGFP1載體含有AcGFP1報告基因,綠色熒光蛋白AcGFP1與EGFP相比,亮度更高,細胞毒性更低;
含內(nèi)部核糖體結(jié)合位點(IRES)元件,目的基因與報告基因以雙順反子的形式轉(zhuǎn)錄,分別翻譯。
產(chǎn)品目錄號: 632435
穩(wěn)定性: 穩(wěn)表達 或 瞬表達
組成型/誘導(dǎo)型: 組成型
病毒/非病毒: 非病毒

pIRES2-AcGFP1載體質(zhì)粒圖譜和多克隆位點信息

pIRES2-AcGFP1載體圖譜



pIRES2-AcGFP1多克隆位點

pIRES2-AcGFP1載體簡介

pIRES2-AcGFP1 contains the internal ribosome entry site (IRES; 1, 2) of the encephalomyocarditis virus (ECMV) between the MCSand theAequorea coerulescens green fluorescent protein (AcGFP1) coding region.This permits both the gene of interest (cloned into the MCS) and the AcGFP1 gene to be translated from a single bicistronic mRNA. pIRES2-AcGFP1 is designed for the efficient selection (by flow cytometry or other methods) of transiently transfected mammalian cells expressing AcGFP1 and the protein of interest.This vector can also be used to express AcGFP1 alone or to obtain stably transfected cell lines without time-consuming drug and clonal selection.AcGFP1 is a green fluorescent protein (GFP) from Aequorea coerulescens. (Excitation maximum = 475 nm; emission maximum = 505 nm). AcGFP1 contains silent mutations that create an open reading frame comprised almost entirely of preferred human codons. These changes increase the translational efficiency of the AcGFP1 mRNA and consequently the expression of AcGFP1 in mammalian and plant cells.
The MCS in pIRES2-AcGFP1 is between the immediate early promoter of cytomegalovirus (PCMVIE) and the IRES sequence. Additional features include SV40 polyadenylation signals downstream of the AcGFP1 gene to direct proper processing of the 3' end of the bicistronic mRNA. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40T antigen. A neomycin-resistance cassette (Neor), consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the herpes simplex virus thymidine kinase (HSV TK) gene, allows stably transfected eukaryotic cells to be selected using G418 (3). A bacterial promoter upstream of this cassette expresses kanamycin resistance in E. coli. The pIRES2-AcGFP1 backbone also provides a pUC origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production. 

pIRES2-AcGFP1載體序列

hz-8083R CCDC124  卷曲螺旋結(jié)構(gòu)域蛋白124抗體
hz-8084R CCDC112/MBC1  膀胱癌突變蛋白1抗體
hz-8085R CCDC82  卷曲螺旋結(jié)構(gòu)域蛋白82抗體
hz-8086R CCDC68/se57-1  卷曲螺旋結(jié)構(gòu)域蛋白68抗體(皮膚T**細胞**瘤相關(guān)抗原)
hz-8087R CCDC83  卷曲螺旋結(jié)構(gòu)域蛋白83抗體
hz-8088R CCDC120  卷曲螺旋結(jié)構(gòu)域蛋白120抗體
hz-8089R CCDC23  卷曲螺旋結(jié)構(gòu)域蛋白23抗體
hz-8090R CCDC136/NAG6  鼻咽癌相關(guān)蛋白6抗體
hz-8091R CCDC40  卷曲螺旋結(jié)構(gòu)域蛋白40抗體
hz-3975R AMY2A  胰腺α淀粉酶抗體
hz-8092R CCDC144A  卷曲螺旋結(jié)構(gòu)域蛋白144A抗體
hz-8093R CCDC60  卷曲螺旋結(jié)構(gòu)域蛋白60抗體
hz-8094R CCDC89  卷曲螺旋結(jié)構(gòu)域蛋白89抗體
hz-8095R CCDC114  卷曲螺旋結(jié)構(gòu)域蛋白114抗體
hz-4030R Pancreatic Amylase  胰淀粉酶抗體
hz-8096R CCDC39  卷曲螺旋結(jié)構(gòu)域蛋白39抗體
hz-8097R CCDC30  卷曲螺旋結(jié)構(gòu)域蛋白30抗體
hz-8098R CCDC34  卷曲螺旋結(jié)構(gòu)域蛋白34抗體
hz-8099R CCDC75  卷曲螺旋結(jié)構(gòu)域蛋白75抗體
hz-8100R CCDC65  卷曲螺旋結(jié)構(gòu)域蛋白65抗體
hz-2381R Signal recognition particle  信號識別顆??贵w
hz-8101R CCDC157  卷曲螺旋結(jié)構(gòu)域蛋白157抗體
hz-8102R CCDC90B  卷曲螺旋結(jié)構(gòu)域蛋白90B抗體

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