pEF1/myc-His A載體基本信息
載體名稱: | pEF1/myc-His A |
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質(zhì)粒類型: | 哺乳細(xì)胞表達(dá)載體 |
高拷貝/低拷貝: | 高拷貝 |
啟動(dòng)子: | EF-1α |
克隆方法: | 多克隆位點(diǎn),限制性內(nèi)切酶 |
載體大小: | 6165 bp |
5' 測(cè)序引物及序列: | T7 Fwd:5'd[TAATACGACTCACTATAGGG]3' |
3' 測(cè)序引物及序列: | -- |
載體標(biāo)簽: | His Tag (6x); c-Myc Epitope Tag |
載體抗性: | 氨芐青霉素(Ampicillin) |
篩選標(biāo)記: | Geneticin |
備注: | 易于克隆到其它載體中去;高表達(dá)水平。 |
產(chǎn)品目錄號(hào): | V921-20 |
穩(wěn)定性: | 瞬表達(dá) |
組成型: | 組成型表達(dá) |
病毒/非病毒: | 非病毒 |
All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells,
a choice of selection marker for generating stable cell lines, and an epitope tag for easy
detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector
is available in three reading frames to simplify cloning in-frame with the fusion tag.