pEF1/myc-His A載體基本信息
| 載體名稱: | pEF1/myc-His A |
|---|---|
| 質(zhì)粒類型: | 哺乳細(xì)胞表達(dá)載體 |
| 高拷貝/低拷貝: | 高拷貝 |
| 啟動(dòng)子: | EF-1α |
| 克隆方法: | 多克隆位點(diǎn),限制性內(nèi)切酶 |
| 載體大小: | 6165 bp |
| 5' 測(cè)序引物及序列: | T7 Fwd:5'd[TAATACGACTCACTATAGGG]3' |
| 3' 測(cè)序引物及序列: | -- |
| 載體標(biāo)簽: | His Tag (6x); c-Myc Epitope Tag |
| 載體抗性: | 氨芐青霉素(Ampicillin) |
| 篩選標(biāo)記: | Geneticin |
| 備注: | 易于克隆到其它載體中去;高表達(dá)水平。 |
| 產(chǎn)品目錄號(hào): | V921-20 |
| 穩(wěn)定性: | 瞬表達(dá) |
| 組成型: | 組成型表達(dá) |
| 病毒/非病毒: | 非病毒 |

All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells,
a choice of selection marker for generating stable cell lines, and an epitope tag for easy
detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector
is available in three reading frames to simplify cloning in-frame with the fusion tag.