pPIC6 B載體基本信息
載體名稱: | pPIC6 B, pPIC6B |
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質(zhì)粒類型: | 畢赤酵母表達(dá)載體;分泌表達(dá)載體;胞外表達(dá)載體 |
克隆方法: | 多克隆位點,限制性內(nèi)切酶 |
啟動子: | AOX1 |
載體大小: | 3380 bp |
5' 測序引物及序列: | GACTGGTTCCAATTGACAAGC |
3' 測序引物及序列: | GCAAATGGCATTCTGACATCC |
載體標(biāo)簽: | c-Myc Epitope Tag, His Tag (6x) |
載體抗性: | Blasticidin |
真核篩選標(biāo)記: | Blasticidin |
克隆菌株: | DH5α、TOP10、JM109 |
表達(dá)菌株: | X-33 |
備注: | pPIC6 B載體含E因子,讓你的重組蛋白分泌型表達(dá)。 |
產(chǎn)品目錄號: | V210-20 |
組成型/誘導(dǎo)型: | 誘導(dǎo)型(甲醇) |
pPIC6 A, pPIC6B, & C are Pichia pastoris expression vectors designed for simple selection,high-level expression, and rapid protein purification and detection. The pPIC6 A,B, & C vectors are designed for high-level secreted expression. Both sets of vectors
have the following features:
Blasticidin resistance for direct selection of multi-copy integrants
Inducible AOX1 promoter for high-level expression in Pichia pastoris
C-terminal c-myc epitope for convenient detection with an Anti-myc Antibody
C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelatingresin and detection with an Anti-His(C-term) Antibody
pPIC6 also features:
An é -factor secretion signal for efficient transport of proteins to the medium