pIB/V5-His載體基本信息
| 載體名稱: | pIB/V5-His |
|---|---|
| 質(zhì)粒類型: | 昆蟲表達載體 |
| 克隆方法: | 多克隆位點,限制性內(nèi)切酶 |
| 啟動子: | OplE2 |
| 載體大小: | 3521 bp |
| 5' 測序引物及序列: | OpIE2 Forward:CGCAACGATCTGGTAAACAC |
| 3' 測序引物及序列: | 5'd[CGCAACGATCTGGTAAACAC]3' |
| 載體標簽: | V5 Epitope Tag , His Tag (C-末端) |
| 載體抗性: | 氨芐青霉素 |
| 真核篩選標記: | Blasticidin |
| 克隆菌株: | TOP10、DH5 |
| 宿主細胞(系): | Sf9 、High Five |
| 備注: | -- |
| 產(chǎn)品目錄號: | V802001 |
| 瞬表達/穩(wěn)表達: | 瞬表達 |
| 組成型/誘導(dǎo)型: | 組成型 |
| 病毒/非病毒: | 非病毒 |


The InsectSelect BSD Kit features the pIB/V5-His vector for stable expression of your gene of interest in a variety of common insect cells. This vector has several features that facilitate
expression, analysis, and detection of recombinant protein in insect cells:
? The OpIE2 promoter for constitutive expression
? The Blasticidin resistance gene for rapid selection of stably transfected cells in two weeks
? C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin