pIB/V5-His載體基本信息
載體名稱: | pIB/V5-His |
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質(zhì)粒類型: | 昆蟲表達載體 |
克隆方法: | 多克隆位點,限制性內(nèi)切酶 |
啟動子: | OplE2 |
載體大小: | 3521 bp |
5' 測序引物及序列: | OpIE2 Forward:CGCAACGATCTGGTAAACAC |
3' 測序引物及序列: | 5'd[CGCAACGATCTGGTAAACAC]3' |
載體標簽: | V5 Epitope Tag , His Tag (C-末端) |
載體抗性: | 氨芐青霉素 |
真核篩選標記: | Blasticidin |
克隆菌株: | TOP10、DH5 |
宿主細胞(系): | Sf9 、High Five |
備注: | -- |
產(chǎn)品目錄號: | V802001 |
瞬表達/穩(wěn)表達: | 瞬表達 |
組成型/誘導(dǎo)型: | 組成型 |
病毒/非病毒: | 非病毒 |
The InsectSelect BSD Kit features the pIB/V5-His vector for stable expression of your gene of interest in a variety of common insect cells. This vector has several features that facilitate
expression, analysis, and detection of recombinant protein in insect cells:
? The OpIE2 promoter for constitutive expression
? The Blasticidin resistance gene for rapid selection of stably transfected cells in two weeks
? C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin