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產(chǎn)品資料

pXINSECT-DEST38

如果您對(duì)該產(chǎn)品感興趣的話,可以
產(chǎn)品名稱: pXINSECT-DEST38
產(chǎn)品型號(hào):
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔

簡(jiǎn)單介紹

pXINSECT-DEST38的各批次質(zhì)粒菌株發(fā)貨前均經(jīng)過(guò)嚴(yán)格的多重驗(yàn)證,如存在質(zhì)量問(wèn)題,請(qǐng)?jiān)谑盏疆a(chǎn)品的三個(gè)月內(nèi)通知我司。收到pXINSECT-DEST38后請(qǐng)短暫離心,取2μl轉(zhuǎn)化至對(duì)應(yīng)感受態(tài)中,挑取單克隆重新提取質(zhì)粒后使用。


pXINSECT-DEST38  的詳細(xì)介紹

pXINSECT-DEST38載體基本信息

載體名稱: pXINSECT-DEST38
質(zhì)粒類型: 昆蟲(chóng)表達(dá)載體;Gateway載體
克隆方法: Gateway
啟動(dòng)子: Actin
載體大小: 12419 kb
5' 測(cè)序引物及序列: --
3' 測(cè)序引物及序列: --
載體標(biāo)簽: 無(wú)標(biāo)簽
載體抗性: 氨芐青霉素氯霉素(僅空載體)
真核篩選標(biāo)記: 無(wú)
克隆菌株: DB3.1
宿主細(xì)胞(系): High Five(推薦) 等細(xì)胞系
備注: pXINSECT-DEST38載體與pBmA-neo載體共轉(zhuǎn)染,可以建立穩(wěn)表達(dá)細(xì)胞系;
應(yīng)用Gateway技術(shù),可快速高效將 目的基因片段(尤其適用于目的基因片段>1kb,用傳統(tǒng)的連接酶法效率低的)插入到載體中;
產(chǎn)品目錄號(hào): 12249-025
瞬表達(dá)/穩(wěn)表達(dá): 瞬表達(dá)
組成型/誘導(dǎo)型: 組成型
病毒/非病毒: 非病毒

pXINSECT-DEST38載體質(zhì)粒圖譜和多克隆位點(diǎn)信息

pXINSECT-DEST38 載體圖譜



pXINSECT-DEST38 多克隆位點(diǎn)

pXINSECT-DEST38 載體特征

pXINSECT-DEST38載體簡(jiǎn)介

 pXINSECT-DEST38載體優(yōu)勢(shì):
Using the Express Insect Kit or the Express Insect Vector Set to achieveprotein expression in insect cells has the following advantages:
 Non-lytic, continuous protein expression
 Minimal degradation of your protein of interest as compared to viralexpression systems
 Higher protein expression level achieved than other similar non-viral insectexpression systems
 Choice of transient or stable protein expression
 Adapted for use with the Gateway Technology or traditional restrictionenzyme-mediated cloning

載體特征:
pXINSECT-DEST38 and pXINSECT-DEST39 have the following features:
 The silkmoth (Bombyx mori) cytoplasmic actin gene promoter for constitutive expression of the gene of interest (Johnson et al., 1992)
 The viral transcriptional enhancer, homologous region 3 (HR3) from Bombyx mori nuclear polyhedrosis virus (BmNPV) acts as a constitutive enhancer of the cytoplasmic actin promoter (Lu et al., 1997)
 A viral transcriptional transactivator, BmNPV IE1, to stimulate expression of your gene of interest (Lu et al., 1996)
 N-terminal 6xHis tag fusion proteins to facilitate detection and purification of your protein of interest using pXINSECT-DEST39
 Two recombination sites, attR1 and attR2, for recombinational cloning of the gene of interest from an entry clone
 Chloramphenicol resistance gene located between the two attR sites for counterselection
 The ccdB gene located between the two attR sites for negative selection
 The actin polyadenylation signal for efficient transcription termination and polyadenylation of mRNA
 Ampicillin resistance gene for selection of transformants in E. coli
 The pUC origin for high copy replication and maintenance of the plasmid in E. coli 

pXINSECT-DEST38載體序列

hz-2961R Calsequestrin  肌鈣集蛋白/收鈣素抗體
hz-2962R Syncytin 1/HERV  合胞素1抗體
hz-2963R TASK 3/KCNK9  酸敏感性鉀離子通道蛋白抗體
hz-2964R TSLP  胸腺基質(zhì)**細(xì)胞生成素-1抗體
hz-2965R Myosin-XVIIIb  肌球蛋白XVIIIb抗體
hz-2966R RBPJK/RBP-J  Notch轉(zhuǎn)錄調(diào)控蛋白R(shí)BPJK抗體
hz-2967R Maltose Binding Protein/MBP  麥芽糖結(jié)合蛋白抗體
hz-2968R HPV33 E6  人類**狀瘤病毒33抗體
hz-2969R HPV33 E7  人類**狀瘤病毒33抗體
hz-2970R PHGDH  磷酸甘油酸脫氫酶抗體
hz-2971R PASK/STK39  絲氨酸/蘇氨酸蛋白激酶39抗體
hz-2972R HES1  轉(zhuǎn)錄因子HES-1抗體
hz-2974R Factor VIII  第八因子抗體
hz-2975R Noggin  指(趾)關(guān)節(jié)粘連NOG蛋白抗體
hz-2976R GGT1/CD224  γ谷氨酰轉(zhuǎn)移酶抗體
hz-2977R TAGL2/TAGLN2  細(xì)胞骨架相關(guān)蛋白2抗體
hz-2978R Peroxiredoxin 2/PRXII  硫氧還蛋白過(guò)氧化物酶Ⅱ/巰基抗氧化蛋白抗體
hz-2981R IL-8/CXCL8  白介素8抗體
hz-2982R VIP Receptor 1/VAPC1  血管活性腸肽受體-1抗體
hz-2983R Desmocollin 2  橋粒糖蛋白2/橋粒糖蛋白3抗體
hz-2984R phospho-PLB(Ser16)  磷酸化心臟磷蛋白抗體
hz-2985R ADM2  中介素抗體
hz-2986R CATSPER  陽(yáng)離子通道精子相關(guān)蛋白1抗體
hz-2987R Vitamin D Receptor/VDR  維生素D3受體抗體

滬公網(wǎng)安備 31011702004356號(hào)

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