Bac-to-BacHT Vector Kit 設(shè)計(jì)用于在大腸桿菌 (E. coli) 中生成桿狀病毒載體后，在 Sf9、Sf21 或 High Five 細(xì)胞中表達(dá)和純化帶組氨酸標(biāo)簽的重組蛋白。 pFastBacHT 載體具有以下特點(diǎn)：
用于表達(dá)蛋白質(zhì)的多角體強(qiáng)啟動(dòng)子
用于簡(jiǎn)化克隆的三個(gè)讀碼框
用于輕松純化重組融合蛋白的 N-端 6xHis 標(biāo)簽
用于在蛋白質(zhì)純化后去除組氨酸標(biāo)簽的 TEV 蛋白酶切割位點(diǎn)
引用及參考文獻(xiàn)
DEF6, a Novel Substrate for the Tec Kinase ITK, Contains a Glutamine-rich Aggregation-prone Region and Forms Cytoplasmic Granules that Co-localize with P-bodies.
Hey F, Czyzewicz N, Jones P, Sablitzky F,
J Biol Chem (2012) 287:31073-31084
Product usage: na
Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin.
Dan C; Kelly A; Bernard O; Minden A;
J Biol Chem (2001) 276:32115-32136
Product usage: pFASTBAC/HT and pFASTBAC-1 vectors were used to transfect with Sf9 cells with Cellfectin.
Sp3 Represses Gene Expression via the Titration of Promoter-specific Transcription Factors.
Kennett Sarah B; Moorefield K Scott; Horowitz Jonathan M;
J Biol Chem (2002) 277:9780-9789
Product usage: Baculovirus stocks encoding Sp1, Sp3, M1, and M2 were prepared using the PCR, appropriate primers, and pCMV4-Sp1/flu, pBSK-Sp3/flu, pCR-M1/flu,
and pCR-M2/flu as substrates. PCR reactions employed pGEX-M2 using Taq polymerase. Amplified cDNAs were subcloned in pFASTBacHTA.
