pLVX-Hom-Mem1載體基本信息
載體名稱: | pLVX-Hom-Mem1 |
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質粒類型: | 慢病毒載體;雙順反子載體;信號通路載體;亞細胞定位載體 |
高拷貝/低拷貝: | 高拷貝 |
克隆方法: | 限制性內切酶,多克隆位點 |
啟動子: | CMV |
載體大小: | 8411 bp |
5' 測序引物及序列: | CMV Forward: CGCAAATGGGCGGTAGGCGTG |
3' 測序引物及序列: | -- |
載體標簽: | DmrB |
載體抗性: | 氨芐青霉素 |
篩選標記: | 嘌呤霉素(Puromycin) |
克隆菌株: | Stbl3 |
宿主細胞(系): | 常規(guī)細胞系(293、CV-1、CHO等) |
備注: | 慢病毒載體pLVX-Hom-Mem1表達DmrB融合蛋白;利用信號受體的二聚化作用研究細胞信號通路;DmrB蛋白加了膜定位信號。 |
產品目錄號: | 635073 |
穩(wěn)定性: | 穩(wěn)表達 |
組成型/誘導型: | 組成型 |
病毒/非病毒: | 慢病毒 |
注:
When cloning your gene of interest into the pLVX-Hom-Mem1 vector, make certain that the N-myr-signal is located on the N-terminus of the DmrB fusion protein. The N‐myr‐signal is NOT functional if localized in the middle or at the C‐terminus of a protein of interest. Your gene must either be cloned into the 3’MCS, or if cloned into the 5’ MCS, which is located upstream of the N‐myr signal, you must include a separate N‐myr signal sequence on the forward primer used to amplify your gene of interest.