The pBAD-DEST49 Gateway destination vector is designed for rapid cloning with a Gateway entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli. The pBAD-DEST49 vector features:
The araBAD promoter for tightly regulated expression in E. coli
HP-thioredoxin fusion for improved protein yield and solubility
Enterokinase cleavage site for efficient cleavage of the N-terminal fusion with EKMax
C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
R sites for efficient recombination with any attL-flanked Gateway entry vector
