The pIB/V5-His-DEST vector is designed for rapid lambda phage site-specific recombination with a Gateway entry clone and subsequent expression in Sf9, Sf21, or High Five insect cells. As part of the InsectSelect System, pIB/V5-His-DEST features:
The OpIE2 promoter for constitutive expression
Blasticidin resistance gene driven by the weak GP64 promoter for selection of stable cell lines that have integrated the plasmid into a transcriptionally active site
C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and easy purification with a nickel-chelating resin
