The recombinant human rhinovirus (HRV 3C) protease is used to remove fusion tags from proteins with the HRV 3C cleavage sequence (PreScission Site): Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro or LEVLFQ↓GP.
The recombinant HRV 3C protease is a fusion protein of 22-kDa size, with a his tag. The enzyme retains high activity at 4°C, and can cleave the target protein in solution, in dialysis or on column.

High specificity: recognizes sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue.
Retain high activity at 4°C: cleavage at low temperature preventing instability of target protein at high temperature.
Easy removal: His-tagged protease can be easily removed after cleavage using a variety of solid supports with immobilized metal chelates.
Lyophilized form: increases the stability and shelf life during storage and shipping, and decreases the shipping cost.
Flexible: HRV 3C protease can cleave the target protein in solution, in dialysis or on column.
Cost effective (cost saving): >50% cost saving, lower price, double size.