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SMAD1 antibody

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產(chǎn)品名稱: SMAD1 antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

SMAD1 antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。SMAD1 antibody嚴格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用:WB、細胞**熒光、**組化、流式細胞術(shù)、ELISA、CHIP、IP和peptide array。


SMAD1 antibody  的詳細介紹

SMAD1 antibodyProduct Profile

Product Name Rabbit anti-SMAD1 polyclonal antibody - middle region
Antibody Type Primary Antibodies
Immunogen
The immunogen for anti-SMAD1 antibody: synthetic peptide directed towards the middle region of human SMAD1

SMAD1 antibodyKey Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB

Species Reactivity

BovineDogHorseHumanMousePigRabbitRat

Concentration 1mg/ml
Purification Affinity purified

SMAD1 antibodyTarget Information

Gene Symbol SMAD1
Gene Synonyms
BSP1
JV4-1
JV41
MADH1
MADR1
BSP-1
More 
Gene Full Name SMAD family member 1
Gene Summary
SMAD1 belongs to the SMAD family. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1 mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, SMAD1 can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of SMAD1 forms a complex with SMAD4, which is important for its function in the transcription regulation. SMAD1 is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
More 
Alternative Names
BSP1
JV4-1
JV41
MADH1
MADR1
BSP-1
More 
Molecular Weight(MW) 52kDa
Sequence 465 amino acids

SMAD1 antibodyDatabase Links

Entrez Gene 4086
SwissProt ID Q15797
Protein Accession NP_005891

SMAD1 antibodyApplication

  • Immunoblot

    Lane 1: 5ug of transfected 293T lysate (SMAD1) 
    Lane 1: 5ug of transfected 293T lysate (SMAD2) 
    Lane 1: 5ug of transfected 293T lysate (SMAD3) 
    Lane 1: 5ug of transfected 293T lysate (SMAD4) 
    Lane 1: 5ug of transfected 293T lysate (SMAD5) 
    Lane 1: 5ug of transfected 293T lysate (SMAD6) 
    Lane 1: 5ug of transfected 293T lysate (SMAD7) 
    Lane 1: 5ug of transfected 293T lysate (SMAD8) 
    Lane 1: 5ug of transfected 293T lysate (GFP)
    Primary Antibody Dilution: 
    1:1000
    Secondary Antibody: 
    Goat anti-Rabbit IgG HRP Conjugated
    Secondary Antibody Dilution: 
    1:10,000
    Gene Name: 
    SMAD1
    Submitted by: 
    Amanda Urick, Medical College of Wisconsin

  • Western blot

    0.2-1 ug/ml
    Positive Control: MCF7 cell lysate

Additional Information

Form Liquid
Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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