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產(chǎn)品資料

c-Myc Antibody

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產(chǎn)品名稱: c-Myc Antibody
產(chǎn)品型號(hào):
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

c-Myc Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。c-Myc Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。


c-Myc Antibody  的詳細(xì)介紹

c-Myc AntibodyProduct Profile

Product Name c-Myc Antibody (C-19)
Antibody Type Tags Antibodies
Modification Notes c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.

c-Myc AntibodyKey Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIPIFIHCELISA

recommended for detection of c-Myc p67 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine and feline:
Species Reactivity

HumanMouseRat

Concentration 200 μg/ml
Purification Affinity purified

c-Myc AntibodyTarget Information

Alternative Names
epitope mapping within the C-terminus of c-Myc of human origin
Tissue Specificity epitope mapping within the C-terminus of c-Myc of human origin

c-Myc AntibodyDatabase Links

Entrez Gene 4609

c-Myc AntibodyApplication

  • Application

    Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.

  • Application

    Western blot analysis of c-Myc expression in non-transfected: (A) and mouse c-Myc transfected: (B) 293T whole cell lysates.

  • Application

    Western blot analysis of c-Myc expression in Jurkat whole cell lysate.

  • Application

    Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse uterus tissue showing nuclear localization.

Application Notes
recommended for detection of c-Myc p67 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine and feline:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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